The scheme was first devised in 1574 by Andrew Perne, Master of Peterhouse, who proposed that a stream be diverted from Nine Wells chalk springs through the town and the King's Ditch to improve sanitation.
The design was revived by James Montagu, Master of Sidney Sussex College and built at the expense of the University and town.
From its source in chalkland springs at Nine Wells, near Great Shelford, this stream flows east of Trumpington under bridges on Long Road and Trumpington Road until reaching Coe Fen, where it forms the first tributary of the River Cam above Cambridge.
The stream widens as it skirts the western boundary of the University Botanic Gardens until passing under Bateman Street.
In the last portion before the conduit head at Lensfield Road, where it divides into four branches, the channel flows between Brookside and Trumpington Street through gardens enclosed by 19C wrought iron railings.
Following a fire in 1849, the Market Square was redeveloped and in 1855 a Gothic Revival gabled fountain was erected (marked on many contemporary maps as "lavatory" as there were underground public conveniences here) and the original fountain was partly moved to form the monument in Lensfield Road, and the remainder demolished.