Holštejn is a municipality and village in Blansko District in the South Moravian Region of the Czech Republic, in the historical region of Moravia.

Holštejn lies approximately 15 kilometres (9 mi) north of Blansko, 24 kilometres (15 mi) north of Brno, and 178 kilometres (111 mi) south-east of Prague.

It was a castle and a Market town.

In 1550, Holštejn was described as abandoned and only one mill survived.

This South Moravian Region location article is a stub.