Holmes Herbert (born Horace Edward Jenner; 30 July 1882 – 26 December 1956) was an English character actor who appeared in Hollywood films from 1915 to 1952, often as a British gentleman.

He was the first son of Edward Henry Jenner (stage name Ned Herbert), who worked as an actor and comedian in the British theatre.

Holmes Herbert never made a film in his native country but managed to appear in 228 films during his career in the U.S., beginning with stalwart leading roles during the silent era and numerous supporting roles in many classic Hollywood films of the sound era, including Captain Blood (1935), The Charge of the Light Brigade (1936), The Life of Emile Zola (1937), The Adventures of Robin Hood (1938), and Foreign Correspondent (1940).

Herbert is perhaps best known for his role as Dr. Jekyll's friend Dr. Lanyon in Dr. Jekyll and Mr. Hyde (1931), and made something of a career in horror films of the period, appearing in The Terror (1928), The Thirteenth Chair (1929 and 1937), Mystery of the Wax Museum (1933), The Invisible Man (1933), Mark of the Vampire (1935), Tower of London (1939), The Ghost of Frankenstein (1942), The Undying Monster (1942), The Mummy's Curse and The Son of Dr. Jekyll (1952).

His third wife, Agnes Bartholomew, died in 1955, leaving Herbert a widower.