Its main campus in Philadelphia is in the northeastern section of the city and it has a satellite location in Newtown, Bucks County.

Holy Family is the youngest of the four Catholic universities in the city of Philadelphia; the others are Saint Joseph's, La Salle, and Chestnut Hill College.

HFU is a member of NCAA Division II and the Central Atlantic Collegiate Conference (CACC), which is composed of 13 colleges and universities located in Connecticut, Delaware, New Jersey, New York and Pennsylvania.

Holy Family's women's teams are basketball, cross-country running, lacrosse, soccer, softball, indoor and outdoor track & field and volleyball.

Holy Family's men's teams consist of baseball, basketball, cross country, lacrosse, soccer, and indoor and outdoor track & field.