[8] The Holy Spirit High School Spartans[4] compete in the National Division of the Cape-Atlantic League, an athletic conference comprised of public and private high schools in Atlantic, Cape May, Cumberland and Gloucester counties that operates under the aegis of the New Jersey State Interscholastic Athletic Association.

The 2007 team finished the season with a record of 12-0 and won the Non-Public, Group III state sectional championship with a 26–13 win over Immaculata High School.

[20] In the 1970s through the late 1980s, Holy Spirit made a name for itself in boys' rowing, winning several national championships and competing several times at the Henley Regatta on the River Thames by the town of Henley-on-Thames, England.

The girls eight-person rowing team won the Peabody Cup at the 2003 Henley Women's Regatta, defeating the Kingston School of England.

Their coach, Joseph Welsh Jr., was a member of the boys team at Holy Spirit when it won a similar championship at Henley in 1976.

[29] The 2005 softball team won the Non-Public, South B state sectional championship with a 10–6 win over St. Joseph High School in the tournament final.

[33] In 2016, the girls' soccer team won the South Jersey Non-Public B final against Our Lady of Mercy Academy 4–1 after losing the previous year to Moorestown Friends.

[35] The girls' swimming team won the Non Public A state championship in 2007 with a 102–68 win against Red Bank Catholic High School.

[31][36] The 2007 girls' tennis team took the South A state sectional championship with a 3–2 win over Notre Dame High School in the tournament final.