The church is under the jurisdiction of the Greek Orthodox Archdiocese of America and is locally administered by the Metropolis of Boston.

Holy Trinity was the first church built for a Greek Orthodox congregation in the United States.

The church was built because of the growing population of Greek immigrants in Lowell at the time.

The building takes the shape of a cross, like most traditional Greek Orthodox Churches.

The structure is Lowell architect Henry L. Rourke's interpretation of the Hagia Sophia in Constantinople.