Hopewell Presbyterian Church and Hopewell Cemetery is a historic Presbyterian church and cemetery located at 5314 Old River Road in Florence, South Carolina.

The two-story, frame, Greek Revival-style church was completed in 1842.

It features a pedimented front gable end and two-story portico.

The cemetery, in use since the late-18th century, occupies a three-acre site where the original Hopewell Presbyterian Church stood.

Inside the cemetery is the church's early Session House.