Hopkins and Brother Store is a historic commercial building located at Onancock, Accomack County, Virginia.

The building features corner pilasters, a bracketed cornice, and one "Gothic" window in the attic.

The business served as one of the commercial and maritime trading centers of the Eastern Shore.

Detailed records of the store exist from 1839 to 1965 and have been donated to the Virginia Historical Society.

This article about a property in Accomack County, Virginia on the National Register of Historic Places is a stub.