As of November 19, 2023[update], four Toronto Transit Commission (TTC) streetcar routes either pass through or terminate at Humber Loop.

At the first Humber Loop, riders could transfer to the Mimico radial line to continue westward to Long Branch and Port Credit.

Riders had to change streetcars at Humber Loop to travel between downtown and Long Branch.

[1] Construction of the Gardiner Expressway required the demolition of the bridge over the railway corridor south of Roncesvalles Avenue.

[1] Because of the construction of the Gardiner Expressway, streetcar tracks were relaid along the Queensway in a centre reservation to the current Humber Loop with a connection to Lake Shore Boulevard via a tunnel under the railway corridor and a highway.

As of June 23, 2019[update], the section west towards Long Branch Loop operates mainly with shorter Canadian Light Rail Vehicles (CLRVs), while the section east to Neville Park Loop operates using mostly low-floor accessible Flexity Outlook streetcars.

At Humber Loop, there were several construction tasks including replacing track and overhead, building a new substation, making platforms accessible to accommodate Flexity streetcars and replacing the passing siding on the west-to-east loop with a stub siding.

[16] The TTC plans to relocate the loop to Park Lawn Road where it will better serve development growth in the Humber Bay Shores area.