Humphrey Middlemore, OCart (died 19 June 1535) was an English Catholic priest and Carthusian hermit, who was executed for treason during the Tudor period.

He is considered a martyr by the Catholic Church, and, along with other members of his religious order to meet that fate, was beatified by Pope Leo XIII on 9 December 1886.

The Crown was at first anxious to secure the public acquiescence of the monks of the London Charterhouse in this matter, since they enjoyed great prestige for the austerity and sincerity of their mode of life.

[2] His efforts left them unmoved, and, after debating with them, he obtained authority from Thomas Cromwell to arrest three leading monks of the London house: Middlemore, as the vicar, and with him Doms William Exmew and Sebastian Newdigate.

All three were thrown them into prison, where they were treated with cruelty, being bound to posts with chains round their necks and legs, and compelled to remain thus day and night for two weeks.