The advantageous location on the border of three countries made Hustopeče an important economic centre with markets.

[3] In 1531, Anabaptists led by Jakob Hutter came into the town from Tyrol and Carinthia, and founded a Hutterite community.

[4] Hustopeče was badly damaged during the Thirty Years' War During this period, the acreage of vineyards fell to 10% of its original state.

On 18 July 1894, Hustopeče received access to a Lokalbahn branch line to Šakvice and the Emperor Ferdinand Northern Railway from Vienna to Prague.

After the Munich Agreement in 1938, it was occupied by Nazi Germany and incorporated into Reichsgau Niederdonau as one of the municipalities in Sudetenland.

After World War II, Hustopeče returned to Czechoslovakia and the remaining German-speaking population was expelled according to the Beneš decrees.

Despite efforts to preserve the valuable remaining part, it was decided to demolish the entire building.