An online anthology comic was serialized on Hobby Japan's media website Hobby Channel from June 1, 2010, and sold two volumes as of June 2011; a manga adaptation illustrated by Junichi Iwasaki began serialization in the November 2010 issue of Monthly Comic Alive; and another manga adaptation by Tatara Yano began serialization in Hobby Japan's online manga magazine Comic Dangan on December 23, 2011.

At Anime Expo 2010, Hobby Japan announced that they are planning to release the light novels in North America in the near future.

The series takes place in an alternate version of Japan called Great Japan (大日本国, Dai Nippon-koku), in an alternate timeline where the Tokugawa shogunate remained active and has remained semi isolated from the rest of the world after their defeat by American forces in World War II.

The story takes place at Buou Academic School (武應学園塾, Buō Gakuen-juku), an academy located at the base of Mount Fuji where children of military families train to become samurai warriors.

[12] A second manga adaptation, called Hyakka Ryōran: Seven Spears (百花繚乱 セブン・スピア, Hyakka Ryōran: Sebun Supia), illustrated by Tatara Yano, began serialization Hobby Japan's online manga magazine Comic Dangan on December 23, 2011.

The show was hosted by Aoi Yūki and Minako Kotobuki, the voices for Jubei Yagyu and Sen Tokugawa, respectively.

Certain episodes featured guest voices from the anime series, and listeners can send in comments of the show on the air.

[14] An anime adaptation produced by Arms and directed by KOBUN was announced on the fourth volume of the light novels.

Simulcasts were provided in North America by Anime Network on their video portal, and in Australia and New Zealand by Madman Entertainment.

[15] Six DVD and Blu-ray volumes were released by Media Factory between November 25, 2010 and April 28, 2011, each containing an OVA short called Hyakka Ryōran Samurai Girls: Blushing Maidens in the Pact (百花繚乱 サムライガールズ 〜乙女♥嬉し恥ずかし将士の契り〜, Hyakka Ryōran Samurai Gāruzu ~Otome Ureshi Hazukashi Shōshi no Chigiri~) and a voiced 4-koma illustrated by Chiruo Kazahana.

[17] Samurai Girls is licensed in North America by Sentai Filmworks, and distributor Section23 Films released the series with an English dub (produced by Seraphim Digital) on August 23, 2011 on DVD and Blu-ray.

by Miyuki Hashimoto and the ending theme is "Kekka, Guuzen de Gozasourou" (結果、偶然でござ候) by Yūki, Kotobuki and Kugimiya.

!-) was released by Lantis on December 8, 2010, featuring songs sung by Yū Kobayashi, Saori Gotō, Aoi Yūki, Rie Kugimiya, and Minako Kotobuki.