Managed fires have been used to encourage recovery of wild plants and animals, and interior fences were removed to allow wildlife to move freely.

In 2007, Tompkins Conservation established a rewilding program with nonprofit partners to reintroduce several native animals that had been extirpated from the area during the 20th century.

Species reintroduced include the giant anteater (Myrmecophaga tridactyla), collared peccary (Dicotyles tajacu), South American tapir (Tapirus terrestris), Pampas deer (Ozotoceros bezoarticus), bare-faced curassow (Crax fasciolata), red-and-green macaw (Ara chloropterus), and red-legged seriema (Seriema cristata).

[7] Rewilding Argentina has also reintroduced the giant river otter (Pteronura brasiliensis) and the ocelot (leopardus pardalis) to the park.

Many animals did not survive the fire, since due to the drought there was not much water or unburned patches to seek refuge in.