It is known that, at the end of the 15th century, the church was connected to the Palace of Jorge de Lencastre, Master of the Order of Santiago and Duke of Aveiro.
[1] Between 1513 and 1520 the medieval church was rebuilt by order of King Manuel I with the help of the local population and the Master of Santiago.
In 1531 a strong earthquake struck Setúbal and the Church of São Julião was damaged; the building was considerably modified in Mannerist style and reinaugurated in 1570.
More notable is the decorated lateral portal, a nice Manueline work with twisted columns imitating ropes, vegetal motifs and trilobed arches.
The side walls are decorated with 18th-century blue-and-white azulejos (ceramic tiles), depicting scenes from the life of Julian and Basilissa.