Infante Alfonso of Spain, Prince of the Two Sicilies, Duke of Calabria (30 November 1901 – 3 February 1964) was one of two claimants to the title of the head of the House of Bourbon-Two Sicilies from 1960 until his death in 1964.

Alfonso was the son of Prince Carlos of Bourbon-Two Sicilies (1870–1949) and his wife, María de las Mercedes, Princess of Asturias (1880–1904).

Alfonso's mother was María de las Mercedes, Princess of Asturias, but she died in childbirth in 1904.

Alfonso XIII, King of Spain was unmarried at the time so as the Princess of the Asturias's eldest son, the young infante became heir-presumptive to the Spanish crown, though, unlike his mother, he never held the title of Prince of Asturias.

Alfonso married Princess Alice of Bourbon-Parma (1917–2017), his second cousin, daughter of Elias, Duke of Parma and Piacenza and his wife Archduchess Maria Anna of Austria, on 16 April 1936 in Vienna, Austria.