North of Perry, Iowa 144 enters Boone County for just under 5 miles (8.0 km).

[1] The Boone County stretch of Iowa 144 is predominantly east–west, while the rest of the highway is north–south.

In Greene County, Iowa 144 turns to the northwest, along an abandoned railroad line, and goes through Rippey.

[1] At Grand Junction, Iowa 144 crosses the main Union Pacific Railroad line before intersecting U.S. Highway 30 on the northern side of town.

North of Grand Junction, Iowa 144 continues north through Dana and passes near Paton before ending at Iowa Highway 175 halfway between Gowrie and Harcourt.