They are distinguished from other grasslands of southeastern Australia because they are frequently dominated by Lomandra species (Iron-grasses) which are tussocks in the Asparagaceae[note 1] family, rather than actual grasses.

The distribution of the Iron-grass Natural Temperate Grassland is located within various Aboriginal Nations, including lands of the Ngarrindjeri, Peramangk, Kaurna, Narrunga, Nukunu and Ngadjuri people.

Wildflowers and forbs include Acanthocladium dockeri, Caladenia colorata, Dodonaea procumbens, Eryngium ovinum, Swainsona behriana, Arthropodium strictum, Triptilodiscus pygmaeus, Chrysocephalum apiculatum, Convolvulus erubescens, Goodenia pinnatifida, Bulbine bulbosa, Einadia nutans, Wurmbea dioica, Velleia paradoxa, Swainsona behriana, Vittadinia gracilis and Wahlenbergia luteola.

[1] Mammals, birds, reptiles, insects, spiders and other invertebrates influence the necessary ecological procedure of the grassland, with Macropus fuliginosus, Chalinolobus gouldii, Chalinolobus morio, Tadarida australis, Dromaius novaehollandiae, Aprasia pseudopulchella, Aprasia pseudopulchella, Tiliqua adelaidensis, Pedionomus torquatus and Eolophus roseicapillus being the most recorded species.

Birds include Cincloramphus cruralis, Corvus mellori, Pedionomus torquatus, Aquila audax, Cracticus tibicen, Ocyphaps lophotes, Phaps chalcoptera, Coturnix pectoralis and Turnix velox.