Isabella[1] of Burgundy (1270 – August 1323), Lady of Vieux-Château, was the second and last Queen consort of Rudolf I of Germany.

He was born in 1266 to the later Robert III, Count of Flanders and his first wife Blanche of Sicily.

He was succeeded as Duke of Austria by his co-ruling sons Albert I and Rudolph II.

She returned to the Court of Burgundy and was granted the title of Lady of Vieux-Château on 20 November 1294.

She is not to be confused with another Isabella of Burgundy who married with Pierre IX de Chambly, Lord of Neaufles.