The University of Isfahan (UI) (Persian: دانشگاه اصفهان, romanized: Dāneshgāh-e Esfahān) is a state-operated university located in Isfahan, Iran.

The decision to establish the university was an idea developed by a number of doctors at Amin Hospital in 1939.

Eventually, the board of directors of the hospital approved a plan to establish Isfahan Higher Medical Institute in 1946.

In September of the same year, an ad signed by lecturer Dr. Jelveh, then caretaker of Isfahan Province Health and Medical Treatment Department, was published in local newspapers calling on prospective students to enroll in the new institute.

The first classes were held at Sadi High School (now Soureh Institute).