[2] With one pathway for qualification being one highest ranked NPCs on the UCI Para-Cycling male and female Nations Ranking Lists on 31 December 2014, Italy qualified for the 2016 Summer Paralympics in Rio, assuming they continued to meet all other eligibility requirements.

Italy earned a qualifying spot at the 2016 Summer Paralympics in this sport following their performance at the 2015 ICF Canoe Sprint & Paracanoe World Championships in Milan, Italy where the top six finishers in each Paralympic event earned a qualifying spot for their nation.

Veronica Yoko Plebani earned a third spot for Italy after finishing fifthin the women's KL3 event.

Italy qualified for the 2016 Games under this criterion in the AS Men's Single Sculls event with an eighth-place finish in a time of 04:58.860.

Italy qualified a second boat in the AS Women's Single Sculls event with a sixth-place finish in a time of 05:50.920.

At this competition, Massimo Croci earned a qualifying spot for their country in the R3 - Mixed 10m Air Rifle Prone SH1 event.

Pamela Novaglio earned a qualifying spot for their country at this competition in the R5 Mixed 10m Air Rifle Prone SH2 event.