[5][6] At the age of 18, Miko returned to the United States and landed her first major film role as Cammie in Coyote Ugly (2000).

[8][9] Her character Cammie works as a bartender at Coyote Ugly and becomes friends with a new employee Violet, played by Piper Perabo.

[14] The following year, she appeared as a seductive nightclub singer in Dark Streets (2008), an adaptation of the play of the same name by Glenn M. Stewart.

She also appeared in the crime thriller Double Identity (2009) in a lead role and in the big-budget remake Clash of the Titans (2010) as Athena.

[15] In 2012, Miko appeared opposite Danny Huston and Sienna Miller in the period drama Two Jacks, directed by Bernard Rose, and in the South Korean dance film Make Your Move in 2013.

Miko appeared in Billy Idol's music video for "Can't Break Me Down", and also had a supporting role in Step Up: All In, both in 2014.

Also in 2014, she had a small role in the fantasy television series Supernatural, episode "Ask Jeeves" of season 10, where she portrays a shape shifter working for a rich heiress who has died.

In 2015, Miko co-produced the biographical film Desert Dancer, which tells the story of Iranian choreographer Afshin Ghaffarian.