Jēkabpils district (Latvian: Jēkabpils rajons) was an administrative division of Latvia, located in Latgale region, in the country's east.

It was organized into three cities and twenty one parishes, each with a local government authority.

It bordered the former districts of Madona to the north, Aizkraukle to the west, Preiļi and Daugavpils to the east.

On 4 January 2000, the unemployment rate was 10.6% of the economically active population according to the State Employment Board data.

Urban population was 32,800 people, rural 25,000 living in 20 parishes and 2 towns.