Jacksonville is a village in Randolph County, Missouri, United States.

[5] According to the United States Census Bureau, the village has a total area of 0.11 square miles (0.28 km2), all land.

The racial makeup of the village was 98.0% White, 0.7% African American, and 1.3% from two or more races.

25.0% of all households were made up of individuals, and 7.2% had someone living alone who was 65 years of age or older.

19.3% of all households were made up of individuals, and 14.0% had someone living alone who was 65 years of age or older.

About 2.3% of families and 7.6% of the population were below the poverty line, including 1.6% of those under the age of eighteen and 42.9% of those 65 or over.