The Jacksonville Dolphins football program was the intercollegiate American football team for Jacksonville University located in the U.S. state of Florida.

The team played its home games at the 5,000 seat D. B. Milne Field in Jacksonville, Florida.

On December 3, 2019, the university announced it would discontinue its football program, effective immediately.

As winners of the Pioneer Football League's South Division, Jacksonville has made one appearance in the Pioneer Football League Championship Game, in 2001.

The Dolphins also shared the Division title with Morehead State in 2004, but the tie-breaker allowed the Eagles to represent the division in the championship game.