Jacobs Hill is a 173-acre (70 ha) open space preserve in Royalston, Massachusetts acquired in 1975 by the land conservation non-profit organization The Trustees of Reservations.

The preserve includes scenic vistas, ledges, woodlands, a northern bog, and Spirit Falls, a 30 foot (9.1 m) waterfall.

Acreage was purchased by the Trustees of Reservations in 1975 out of anonymous funds; additional parcels were acquired in 1978 and 1994.

The preserve is open to fishing, walking, picnicking, hunting (in season), cross country skiing, and hiking.

Jacobs Hill is part of a larger contiguous area of protected open space, connected by the Tully Trail and including the United States Army Corps of Engineers' Tully Lake flood control project, The Trustees of Reservations' Royalston Falls and Doane's Falls properties, and Massachusetts state forest land.