In 1472 he was sharing a workshop with Biagio d'Antonio, and in 1473 he formed a partnership with Filippo di Giuliano that he maintained until his death in 1493.

His most famous such commission is the Morelli and Nerli pair of 1472 (now London, Courtauld Institute Gallery), manufactured by the carpenter Zanobi di Domenico and painted by Sellaio in collaboration with Biagio d'Antonio.

[5] During the German occupation of France, Sellaio's "Madonna and Child, Saint John the Baptist and two angels" was seized in Paris from Austrian refugees Anne and Fritz Unger by a Nazi looting organisation.

A settlement between the Cerruti Foundation and the Arens-Unger family specified that the museum will tell the history of the stolen and recovered work.

[6] The American poet Ezra Pound wrote a poem called Of Jacopo del Sellaio.