After persistent internal divisions, in November 2014, Lambie resigned from the Palmer United Party to sit in the Senate as an independent.

He survived a challenge to his own eligibility, on a different constitutional ground, but refused to step down so as to create a casual Senate vacancy to which Lambie could be appointed.

[7] Lambie was one of just four members of the 46th Parliament of Australia who did not graduate from high school, the others being Julie Collins, Llew O'Brien and Terry Young.

She has since been an advocate for veterans with the Returned and Services League of Australia and involved in fundraising with the Burnie Chamber of Commerce, the Country Women's Association and Rotary.

[11] The Department of Veterans' Affairs (DVA) initially rejected her application for compensation, but subsequently approved it and put her on a military disability pension.

The tribunal's Deputy President, Justice Christopher Wright, concluded that "it is likely that even greater improvement would have been achieved a long time ago if her medical treatments, which were initially funded by the respondent, had not been terminated in 2001".

"[15] In the 2013 federal election, Lambie won Tasmania's sixth Senate seat as a candidate for the Palmer United Party, receiving 6.58% of first preference votes.

[23] In 2018, the High Court ruled that Lambie's running mate Steve Martin would replace her in the Senate, following a recount.

[24] Lambie expected Martin to immediately resign, which would have cleared the way for her to be appointed to fill the resulting casual vacancy and return to the Senate.

A party spokesman contended that Tasmanians intended for Lambie to hold the seat, and there was "an opportunity for that vote to be restored" if Martin resigned.

[28][29] In 2020, Lambie opposed the Liberal Party's university reform bill due to her belief it would harm the mental health and economic opportunities of low-income students.

[30][31][32] In February 2016, Lambie raised the matter of former soldiers who claim to have suffered abuse, calling for an inquiry into cover-ups and Lieutenant General David Morrison's involvement.

[33] In August 2014, Lambie expressed her belief that China could invade Australia: "If anybody thinks that we should have a national security and defence policy which ignores the threat of a Chinese Communist invasion – you're delusional and got rocks in your head ...

[35] Lambie has made comments suggesting her support for potential reintroduction of national service, stating "It's time to teach [our youth] some respect, loyalty and honour.

"[15] In October 2015 she declared her opposition to the China–Australia Free Trade Agreement, saying she considers the Chinese government to be "push[ing] totalitarian ideologies", "anti-democratic" and "a bully, thief, liar and international human rights abuser".

[38] In October 2016, she called for a pre-emptive pardon for any defence personnel accused of war crimes against the Taliban or Islamic State, on the grounds that Taliban and Islamic State fighters were not entitled to the protection of the rules of war or international human rights because of their "subhuman behaviour and vile, disgusting culture and ideology".

[40] She also attacked supporters of Islamic sharia law, describing them as "maniacs and depraved humans" who will not stop committing "cold-blooded butchery and rapes until every woman in Australia wears a burka".

[41] In February 2017, she introduced a private member's bill which would amend the Criminal Code Act 1995 to make it illegal to wear full face coverings in public places when a terrorism threat declaration is in force, unless it was necessary for certain purposes.

[44] Following her involvement in the TV show Go Back to Where You Came From in 2018 where she was placed in a Syrian warzone, Lambie shifted towards a pro-refugee stance, stating that "the discussion [about accepting more refugees] needs to be on the political table".

[45] On 22 August 2024, Lambie was accused by fellow Senator Mehreen Faruqi of anti Muslim sentiment during a parliamentary debate over Australia's granting of visas for Palestinians.

"I had to sit here and listen to Senator (Jacqui) Lambie attack and vilify Muslims and she couldn't even pronounce my name", she claimed on SBS News.

[46] In October 2013 she criticised the Australian Greens, accusing them of having "destroyed all hope in Tasmania" and saying that the party should be subject to a Senate inquiry over the state's high unemployment rate.

[52] Lambie criticised the Government's plan in a Dissenting Report, noting that "The families of veterans who have taken their own lives support a Royal Commission.

In 2002, the Administrative Appeals Tribunal had ruled that descent from Margaret Briggs was sufficient to meet the Aboriginal ancestry requirements for ATSIC elections.

[75] Lambie's claims of Indigenous descent have been questioned by several sources, including Australian Story, the Tasmanian Pioneer Index, and Clyde Mansell, chairman of the Aboriginal Land Council of Tasmania.