Jan Macháček (born 15 February 1972 in Prague) is a former Czech rugby player.
He is one of arguably the two most famous Czech rugby players, along with Martin Jágr.
He played for Newport RFC and Pontypridd RFC in Wales, Sale Sharks in England, Clermont Auvergne in France as well as the Barbarians.
The first team he played for was the Czech Slavia Prague.
He played his final match on 16 December 2009 in a 17–5 win against Hong Kong in Prague, aged 37 years.