Completed and commissioned in December 1942, she served in World War II, operating in the Solomon Islands.

[2] For surface running, the boats were powered by two 500-brake-horsepower (373 kW) diesel engines, each driving one propeller shaft.

[1] Upon commissioning, Ro-107 was attached to the Sasebo Naval District and was assigned to the Kure Submarine Squadron for workups.

[5] She put back to sea on 27 May 1943 to begin her second war patrol, again bound for the waters east of Guadalcanal.

[5] On 1 August 1943, the Imperial Japanese Navy declared her to be presumed lost in the Solomon Islands with all 42 men on board.

[5] Some historians have credited the United States Navy submarine chaser USS SC-669 with sinking Ro-107 off the entrance to the harbor at Espiritu Santo in the New Hebrides in May 1943, but Ro-107 was active until July 1943.

[5] The U.S. Navy destroyer USS Taylor (DD-468) erroneously received credit for sinking a Japanese submarine in Kula Gulf on 12 July 1943 and Taylor′s victim has been widely identified by historians as Ro-107, although some historians have claimed Taylor sank I-25.