Jayshree was born into a Gaud Sarswat Brahmin family at Kanasgiri (Sadashivgad) near Karwar in the Uttara Kannada district of Karnataka, India.

[6] She married Bal Dhuri, a theatre actor best known for his portrayal of Dashratha in Ramanand Sagar's TV serial, Ramayana (where Jayashree herself played his wife, Kaushalya).

Later, well known Marathi film Director Dinkar D. Patil cast her in a small role with dance in his Marathi film Disat Tasa Nasat, opposite Raja Gosavi.

She eventually became one of the most successful and prolific heroines in the history of the Marathi film industry.

Her directorial efforts include Saasar Maher and Ashi Asavi Saasu.