Jefferson is a borough in York County, Pennsylvania, United States.

According to the United States Census Bureau, the borough has a total area of 0.6 square miles (1.6 km2), all land.

Jefferson is primarily covered by Spring Grove Ambulance service, which occasionally has Advanced Life Support (ALS) capabilities for EMS needs.

The Jefferson Fire Department also has a Quick Response Service (QRS), that responds to provide Basic Life Support (BLS) care to patients until the arrival of a transport unit.

Other organizations that may provide EMS to this jurisdiction if Spring Grove is unavailable, including when they lack ALS coverage, includes the Lineboro Volunteer Fire Department (ALS), Community LifeTeam EMS, Pleasant Hill Fire Company (BLS), and other surrounding organizations.