A 1942 newspaper article reported, "Jennifer Holt ... has been signed to a long term contract with Universal.

All but eight of her roles were in Western films in which she appeared opposite cowboy stars such as Lash LaRue, Tex Ritter and Johnny Mack Brown.

[6][7] After making her final film in 1949, in 1950 Holt co-hosted a television show called Panhandle Pete and Jennifer which ran for one season.

During the remainder of the 1950s she made occasional guest appearances on television Western series such as The Gabby Hayes Show and Tales of Wells Fargo starring Dale Robertson.

The marriages that can be confirmed were: Holt was living in Dorset, England in the United Kingdom with her husband, Aylmer Hughes Chamberlain, at the time of her death from cancer at age 76 in 1997.