It currently plays in Serie D. Unione Sportiva Jesina was born on 15 March 1927 by the merger of Juventus Aesina and SS Mazzini Jesi.

In 1933 the club was promoted to the third tier Serie C, a division where the Leoncelli played until the World War II giving the town of Jesi matches against major city teams such as Taranto, Pescara, Venezia, Vicenza, Udinese and Padova.

The swan song of the Latini era comes the next year, when Jesina loses the final of Coppa Italia di Serie C against Virescit Boccaleone.

At the end of the 1990–1991 season, the team lose after penalty kicks a relegation playout and financial crisis sends Jesina another division down returning to the regional championship.

Than a decade of frustration came with many promotion playoffs failures, many times against smaller or rival teams such Cingolana, Centobuchi, Elpidiense Cascinare or Vigor Senigallia.