In 1630 he was sent to Leipzig as a delegate to a convention in behalf of the Augsburg Confession, and in 1645 he took a leading position at the colloquy of Thorn.
He wrote Calvinisimus irreconciliabilis (Wittenberg 1644) as the counterpart to Bishop Joseph Hall's Roma irreconciliabilis, adding an appendix Quae dogmata sint ad salutem creditu necessaria, which is somewhat conciliatory towards the Reformed doctrine of the Lord’s Supper and the personal union.
In the years following Thorn, he became one of the most prominent adversaries of Calixtus, and though Abraham Calovius is more remembered today, many of his contemporaries considered him the leader of German Lutheranism.
According to Ingetraut Ludolphy, he was a born systematician, whose attacks on Calixtus and the other Helmstedt theologians are far superior to most other anti-Helmstedt polemics.
Huelsemann’s principal works are his Brevarium theologiae (Wittenberg, 1640; enlarged with the title, Extensio brevarii theologiae, Leipzig, 1655); Muster und Ausbund gutter Werke (1650); Dialysis apologetica problematic Calixtini (1651); and Der Calixtuinische Gewissenswurm (1653).