Johannes matriculated at the University of Kraków on 19 May 1491, when he studied natural history and astronomy.
He travelled to Vienna, Venice, Padua, Ferrara, Rome, and finally to Bologna, where he studied Greek.
In 1506, he took a position at the newly founded University of Frankfurt as the Professor of Poetry and Rhetoric, alongside Gregor Schmerlin.
In 1514 he opened a Latin school in Cottbus and a year later in Saxon Freiburg, which was headed by Ulrich Rülein von Calw.
In winter 1519, Aesticampianus fell ill and afterwards suffered from shortness of breath.