He also wrote the Icones veterum aliquot ac recentium Medicorum Philosophorumque in 1574, published in Antwerp.

In 1560 he returned to Vienna, settling down as a physician and leading exponent of scientific and cultural knowledge.

Sambucus was appointed the court physician of Emperor Maximilian II, as well as the Imperial Council and Hofhistoriograf.

The library contained numerous previously unknown ancient and contemporary Greek and Latin manuscripts, for instance: Janus Pannonius and Antonio BONFINI, whose texts he partially edited or through his influence supplied patronage to.

He also published several maps and geographical descriptions, e.g. "Hungaria", "Transilvaniae Descriptio" and "Illirium", and sometimes wrote Latin poems.