John Cook (13 April 1805 – 31 March 1892) was a minister at a Presbyterian Church in Canada and educator associated with Quebec.

He served as a minister in the Church of Scotland, and was ordained in December 1835 in Cardross, and designated minister of St. Andrew's Presbyterian Church, Quebec City (under the Colonial Committee) and arrived in Canada East in April 1836.

He had also served as Moderator of the Church of Scotland's Canada Synod in 1838, not long after his arrival.

Cook also promoted education, started schools, and was associated with the early days of both Queen's College at Kingston, Canada West and McGill University.

He was awarded the Doctor of Divinity in 1838 from the University of Glasgow, and the LLD from Queen's in 1880, following his term as Chancellor.