John Darling Jr. (24 January 1852 – 27 March 1914) was a Scottish born South Australian businessman and politician.

Under his control, the company purchased the Eclipse flour mills, Port Adelaide, and the goodwill of J. Dunn and Co. in ???.

He was an active member of the Chamber of Commerce, the Shipowners' Association, and the South Australian Employers' Union.

He was proud of his Scottish heritage; he was a member of the Caledonian Society of South Australia, and its Chief 1904–1907.

He purchased Glenaroua Station in Victoria from Thomas Singleton in 1901, then moved to Carnamah, Western Australia and became the largest landowner in the region, passed to son Arthur.