Sir John Lisle (1610 – 11 August 1664) was an English lawyer and politician who sat in the House of Commons at various times between 1640 and 1659.
He supported the Parliamentarian cause in the English Civil War and was one of the regicides of King Charles I of England.
He was assassinated in a churchyard in Lausanne on 11 August 1664 by Sir James Fitz Edmond Cotter, an Irish soldier and Royalist agent who tracked down regicides and who is said to have used the alias Thomas Macdonnell.
The conduct of the trial, where Judge Jeffreys, presiding, applied intense pressure on the jury to convict, caused much unfavourable comment; and the refusal of King James II to heed pleas for mercy gave rise to a belief that he was taking posthumous revenge on Sir John himself.
Another of John's children, Bridget, married Leonard Hoar, the 3rd President of Harvard College.