John Singleton Millson (October 1, 1808 – March 1, 1874) was an American lawyer and politician who served six consecutive terms as a U.S. Representative from Virginia from 1849 to 1861.

He served as chairman of the Committee on Revolutionary Pensions (Thirty-second Congress).

He is notable as of one of only two Southern Democrats to have voted against the Kansas-Nebraska Act, the other being Thomas Hart Benton.

This article incorporates public domain material from the Biographical Directory of the United States Congress

This article about a member of the United States House of Representatives from Virginia is a stub.