John Thompson (died December 1634) was a noted boy player acting women's roles in English Renaissance theatre.
According to the cast list in the 1623 first edition of Webster's The Duchess of Malfi, Thompson played Julia, the "Cardinals Mis".
He also played Domitia in The Roman Actor (1626), and Honoria in The Picture (1629), both by Philip Massinger, plus Cleonarda in Carlell's The Deserving Favourite (also 1629) and Miniona Undermine in Clavell's The Soddered Citizen (1630).
Thompson fades from view after 1631; but the very patchy records of the era may obscure the fact that he simply continued acting.
The parish records of St. Giles without Cripplegate, which was the home of many theatre people of the day and located near the Fortune Playhouse, note two Thompson daughters in 1631 – the christening of Anne on 16 July and the burial of Lettice on 1 October.