[1] He then designed gardens on the site of St Augustine's Abbey for Lord Wotton in 1615–1623.

[1] He travelled to the Nikolo-Korelsky Monastery in Arctic Russia in 1618 (his own account of the expedition survives in his collection), to the Levant and to Algiers during an expedition against the Barbary pirates in 1620, returned to the Low Countries on Buckingham's behalf in 1624, and finally went to Paris and (as an engineer for the ill-fated siege of La Rochelle) the Île de Ré with Buckingham.

[1] The Ark was the prototypical "Cabinet of Curiosity", a collection of rare and strange objects, that became the first museum open to the public in England, the Musaeum Tradescantianum.

He gathered specimens through American colonists, including his personal friend John Smith, who bequeathed Tradescant a quarter of his library.

[1][4][5] A genus of flowering plants (Tradescantia) was named in honour of the two men by Carl Linnaeus in 1752.