He has appeared in the films Pootie Tang, School for Scoundrels, and Be Kind Rewind, and he has guest-starred on comedy programs such as Curb Your Enthusiasm, Louie, Wonder Showzen, Bob's Burgers and Aqua Teen Hunger Force.

He was also a lead voice actor in several animated comedy programs such as Stroker & Hoop, Freak Show, and Lucy, the Daughter of the Devil.

In 2012, he began a recurring role as Leslie Knope's rival, Councilman Jeremy Jamm in the fifth and sixth seasons of the NBC sitcom Parks and Recreation.

Glaser is the creator and star of the live-action Adult Swim series Delocated, about "Jon", a man in the witness protection program with his own reality show.

Glaser's Delocated character, "Jon" was also featured as a DJ on an Adult Swim-themed radio station in the video game Saints Row: The Third.

In Second City's award-winning revue Pinata Full of Bees, which was directed by Tom Gianas, Glaser sang about the importance of not betraying a friendship by pretending not to have legs in order to play in a wheelchair basketball league.

Glaser created and starred in the Comedy Central web-series Tiny Hands, which has been shown on the channel's short film series Atom TV a number of times.

[5] He was also the bass player in "A Matter of Trust", another comedy music project he was involved in with comedians H. Jon Benjamin, Todd Barry and Tom Shillue.