The grand prince and metropolitan convened a church council, headed by Joseph's brother, the bishop of Rostov, which deposed Serapion and confined him to the Troitse-Sergeev Lavra, where he died in 1516.
[1] Over the next several decades, the monastery became the center of his disciples, or Josephinians, and played a key role in the political and ecclesiastic life of the 16th-century Russia.
The famous inmates included Maximus the Greek (who spent 14 years there), Vassian Patrikeyev, Feodor Kuritsyn, Feodosii, Archbishop of Novgorod (1542–1551) who helped in compiling the Velikaia Mineia Chetii of Makarii and helped draw up documents for the Stoglav Council, Metropolitan Daniel, and Tsar Basil IV.
Joseph Volotsky, Metropolitan Daniel, Archbishop Feodosii, and Malyuta Skuratov are among many notables buried within monastery walls.
After the Assumption Cathedral was constructed in brick in 1486, the great icon-painter Dionisius was summoned to embellish its walls with frescoes.
During the Time of Troubles, Joseph Volokolamsk Monastery was actively engaged in helping the government of Basil IV in his struggle against Ivan Bolotnikov's rebels.