During the siege of the Jewish city of Bethulia by the Assyrians, a widow named Judith (Blanche Sweet) has a plan to stop the war as her people suffer starvation and are ready to surrender.

The widow disguises herself as a harem girl and goes to the enemy camp, where she beguiles a general of King Nebuchadnezzar, whose army is besieging the city.

Judith seduces Holofernes (Henry Walthall), then while he is drunk cuts off his head with a sabre.

The reviews were favorable: Variety, March 27, 1914, wrote: "It is not easy to confess one's self unequal to a given task, but to pen an adequate description of the Biograph's production of Judith of Bethulia is, to say the least, a full grown man's job."

The Moving Picture World, March 7, 1914, described it as: "A fascinating work of high artistry, Judith of Bethulia will not only rank as an achievement in this country, but will make foreign producers sit up and take notice.