She remained at home following the rule Benizi had given her until her mother's death, when Juliana and several companions moved into a house of their own in 1305.

It is said that "she would often fall in to long moments and hours of ecstacy... She was daily caring for the sick in the streets, homes, and in hospitals..." Juliana directed the community of Servite Tertiaries for 35 years and was more of a servant to her subordinates than a mistress.

At this time, unable to receive Holy Communion because of constant vomiting, she requested the priest to spread a corporal upon her chest and lay the Eucharistic host on it.

Pope Benedict XIII recognized the devotion long paid to her and granted the Servites permission to celebrate the feast of the Blessed Juliana.

Pope Clement XII canonized her in the year 1737, and extended the celebration of her feast day (June 19) to the entire Church.