[3][4] She began her acting career shortly after her 1923 graduation from Hollywood High School,[5] and was signed to a contract by Warner Brothers in 1924.

After producer Hal Roach suggested that brunette Marlowe don a blonde wig to match the hair of the lead kid in the series, Jackie Cooper, she was given the part of Miss Crabtree.

In addition to her work in Our Gang, Marlowe appeared in fellow Roach stars Laurel and Hardy's first feature film, Pardon Us.

In an October 1973 notice, Washington's Evening Star newspaper announced that the Superior Court in Los Angeles "returned a verdict of $100 damages to Henry M. Oviatt against June Marlowe, film actress, and her brother, Armour Marlowe, as the outgrowth of a motor car collision," adding that their automobile had "collided with one containing Oviatt and Mrs. Nellie McLaren, who sued for $5,000 each, alleging injuries", and that "Mrs. McLaren was denied damages.

[13] June Marlowe's Our Gang character Miss Crabtree was mentioned in "Buddy", a track on De La Soul's album 3 Feet High and Rising.