[1] Ho attended Queen's College Hong Kong from 1975 to 1979, after which he went to the United Kingdom, where he enrolled at Chelmer Institute of Higher Education and obtained his bachelor of laws degree in 1984.

Ho joined a post-graduate programme at the University of Hong Kong in 1984 and obtained his mandatory practising qualification, the postgraduate certificate in laws (PCLL) in 1986.

[4] He became the vice-president of the Law Society of Hong Kong in June 2005 and was elected president for a one-year term in May 2011, after which he has served as a council member.

[5] In 2017, his legal qualifications in England and Wales, and Singapore was disputed, and the Solicitors Regulation Authority (SRA) was contacted regarding this matter.

[12] Ho first contested a Legislative Council election in 2008, running against Civic Party's Margaret Ng in the Legal functional constituency.

[14] Ho was appointed to Lingnan University council by Chief Executive of Hong Kong Leung Chun-ying in October 2015.

In May 2017, pro-democracy lawyer Kevin Yam published an article urging solicitors not to vote for Junius Ho in the election for the governing council of the Law Society of Hong Kong.

[24] On 6 November 2019, in preparation for the 2019 Hong Kong local elections, Junius Ho ran a campaign event in Tuen Mun.

[29] In May 2017, Ho said that a ruling granting marriage benefits to a gay civil servant could lead to "chaos in society" and co-signed a petition asking the government to appeal the decision.

[30] In April 2018, Ho became the only legislator to vote against the appointments of foreign judges Brenda Hale and Beverley McLachlin to the Court of Final Appeal over their support of same-sex rights, claiming the two opposed traditional family values.

Eddie Chu criticized Ho's position as unclear and absurd, and compared him to Yuan Mu, a Chinese politician who in an interview with Tom Brokaw in 1989 claimed that there were no casualties during the crackdown in Tiananmen Square.

[38] Ho was a leading critic of legal scholar Benny Tai's Occupy Central with Love and Peace which suggested a full-scale occupation protest in the form of civil disobedience to press the Beijing government to make concessions on electoral reform.

Ho submitted a petition, appearing to have the support of over 80,000, urging the university to investigate Tai and organised a rally on 17 September calling for his removal.

"[48] The 22 lawmakers from the pro-democracy camp issued a joint statement condemning Ho's remarks, which read in part: "Ho, as a legislator and lawyer, expressed hate speech involving murder at a public event, crossing the bottom lines of free speech and morality and severely breaching professional conduct.

[52] On 25 April 2018, it was reported that Ho's family business holds the ownership of 120,000 square feet of farmland southwest of the Fanling Golf Course.

Ho had previously voted against a motion to request the government to seize the golf course for housing redevelopment, but did not declare any potential conflict of interests.

[53] On 2 May 2018, lawmaker, Andrew Wan, filed a complaint to the legislative committee accusing Ho of failing to declare two properties that is associated to his family's company, Profit Trade Investment Ltd and other subsidiaries.

They urged LegCo members to impeach Ho, and requested Queen's College Old Boys' Association to suspend his membership.

[60][59] Similarly, Lingnan University students have started a petition calling for Ho to be removed from the institution's governing council.

An online petition requesting that the United States bar Ho and his family from entering the U.S. or acquiring U.S. citizenship achieved over 100,000 signatures.

[59] On 23 July 2019, Ho appeared on an RTHK televised forum alongside fellow New Territories West constituency representative Eddie Chu.

Mo, who is married to English journalist Philip Bowring, later told the council that the comment amounts to racism and sexual harassment.

[70] Ho later claimed that the real issue was not the party, but the government's policy of allowing aircrew from Cathay Pacific to quarantine at home.

[77] The graves of Ho's parents were vandalized during the 2019–20 Hong Kong protests, which is believed to be due to his association with the Yuen Long attacks,[78][79] though the identity of the perpetrators remains unknown.