Following the reintroduction of reserve teams in Italy, Juventus U23 was formed in 2018 and was registered to Serie C, the third level of the Italian league system.

In the 2019–20 season, under coach Fabio Pecchia, Juventus U23 won the Coppa Italia Serie C after defeating Ternana in the final.

[3] Due to the nature of the club as a reserve team, Juventus Next Gen needs to comply to certain regulations, such as being eligible for promotion to Serie A and competing in the Coppa Italia, the major national cup.

The club, and in general FIGC's reserve-team project, has faced a lot of criticism, especially from fans of other Serie C teams due to their perceived lack of history.

[5] Juventus II also took part in the Campionato De Martino [it], another league dedicated to reserve teams, until the competition ceased in 1976; they won the 1959–60 edition.

Juventus U23 were founded on 3 August 2018,[12] following the reintroduction of professional reserve teams in Italian football after over 40 years, and was officially admitted to the Serie C championship.

[13] On 21 August, Luca Zanimacchia became the first scorer of the team's history after scoring the only goal in a 1–0 win over Cuneo in the group stage of the Coppa Italia Serie C.[14] Juventus U23's first game in Serie C was a 2–1 away defeat to Alessandria, with Claudio Zappa scoring the team's first league goal.

[26] Brambilla left Juventus Next Gen at the end of the season[27] and was substituted by former U19 coach and former first-team defender Paolo Montero in mid 2024.

[28] Montero was sacked in the November of the same year, with the team trailing at the penultimate place in relegation zone and with them obtaining one only victory in 14 league matches.

[32] Although there had been an agreement among the two parts shortly after Juventus's reserve team's foundation, Alessandria's fans protested to not share their stadium.

[33] The match was drawn 2–2, with Simone Iocolano scoring a brace for Juventus Next Gen[34] and was seen by 28,572 fans present at the stadium.

Juventus B are going to be harmful and will distort the championship: they are not a newly promoted team but they are certainly going to be strong, we have seen this in women's football that the Bianconeri don't make a bad impression wherever they put their effort".

[50] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.