[1] The hospital is located in the town of Kalongo, Agago District, Acholi sub-region, in the Northern Region of Uganda.

This location lies approximately 160 kilometres (99 mi), by road, northeast of Gulu, the largest city in the sub-region.

[2] Kalongo is located approximately 462 kilometres (287 mi), by road, northeast of Kampala, the capital of Uganda and the largest city in that country.

Giuseppe Ambrosoli, an Italian physician, surgeon and catholic priest, began the transformation of the dispensary into a full-fledged hospital.

Giuseppe Ambrosolio established  St. Mary's School of Midwifery, with a view of someday in the future handing over the care of the patients to local Ugandan staff.